Library Detail Page for Petunia-PP

Short Name:Petunia-PP
Organism:Petunia hybrida
Library Name: Petunia Floral Post Pollination
Total Sequences: 1269 sequences from 1824 clones
Average Sequence Length:559.6 (Standard deviation 124.7)
Type:cDNA Library
Tissue:all floral organs
Development Stage:anthesis
Treatment Conditions:Flowers at anthesis stage were self-pollinated and entire flowers were collected at 0, 5, 10, 24, 36 and 48 hours after pollination from plants grown in standard greenhouses. Total RNA was extracted from each sample, and 100 micrograms of each sample was combined for subsequent poly A+ mRNA selection and cDNA synthesis.
Cloning Host:lambda ZAPII unidirectional
Cloning Kit: Stratagene
Comments
AuthorsKenichi Shibuya, Beverly Underwood, Holly Loucas, William Farmerie, Michelle Jones, David Clark*
Contact InformationDavid G. Clark\ University of Florida\ Environmental Horticulture Dept.\ 1545 Fifield Hall\ Gainesville, FL 32611-0670\ ph: 352-392-1831 x370\ FAX: 352-392-3870\ e-mail: geranium@ufl.edu\ http://hort.ifas.ufl.edu/floriculture/floriculture_bio.htm